Some aspects of immune response to lipopolysaccharides of Providencia rettgeri

Abstract

 LPS  of P.rettgeri was extracted and purified   in our previous study, the present study found that  the injection of 150μg/ Kg of LPS (intramuscularly and subcutaneously) was able to stimulate humoral immune response at both systemic and mucosal levels which was detected by passive haemmaglutination test  and  the mean values of systemic antibody titer (6826.6) was higher than the mucosal (at appendix) antibody titer (512). Some  cytokines concentrations such as IL-2 ,IL-4,IL-6 and IL-1ß were detected using ELISA kits. The study was observed that, the concentrations of IL-2 (3.21 pg/ml ) , IL-4(5.84 pg/ml ) and IL-6 (4831 pg/ml) were increased significantly in LPS immunized animals group compared with control group which were 2.05 , 2.34  and  3121.06   pg/ml for IL-2,IL-4 and IL-6 respectively, while the concentration of IL-1ß (115.7 pg/ml ) was decreased significantly in LPS immunized animal group than control group which was 177.24 pg/ml.