PCR detection of rmpA gene among Klebsiella isolated from wound and burn infections

Authors

  • Ruaa Sh. Al-Rediany
  • Abbas Sh. Al- Muhanna

Abstract

One hundred and twenty five Gram-negative-lactose fermented bacteria grown on MacConkey agar were collected from burn and, wound infections, in Al- sadar Medical City in Al-  Najaf province. The bacterial isolates were identified according to cultural characteristics ,the results revealed that   40(32%) isolates were identified as Klebsiella spp, all of them  40(100%) isolates were belong to K. pneumonia subsp. pneumonia .The capsule of klebsiella isolates were tested .the result revealed all Klebsiella isolates40(100%) were capsulated ,the gene coding capsule in isolates gave positive results. Genotypically were studied carefully using PCR technique , the results pointed out that 35 (87.5%) of bacterial isolated have rmpA  gene coding for producing polysaccharide capsule .

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Published

2013-12-02

How to Cite

Al-Rediany, R. S., & Al- Muhanna, A. S. (2013). PCR detection of rmpA gene among Klebsiella isolated from wound and burn infections. Al-Kufa University Journal for Biology, 5(2). Retrieved from https://journal.uokufa.edu.iq/index.php/ajb/article/view/7784