Molecular Study on Distrbution of Sul-1 and Sul-2 genes among Salmonella enterica causing Enteric Fever

Abstract

This study comprised 200 blood samples taken from patients visited some of Al-Najaf province. The most prevalent species was Salmonella Typhi, where 20  isolates had been identified. Serological typing was done by using both poly and monovalent antisera, which revealed that all isolated Salmonella was belong to serovar Typhi. DNA was extracted from all Salmonella Typhi isolates. Extracted DNA was used in Polymerase Chain Reaction technique. Three pairs of primers was used to detect the presence of targeted genes (two for sul-1 gene, and one for sul-2) . Data showed the occurrence of sul-1 gene the first sequence in 15% of total , while second sequence gave no positive result. Sul-2 gene on the other hand , showed 50% positivity.