Genetic Fingerprint of Some Cucurbita pepo (Summer squash) Genotypes Using Molecular and Biochemical Techniques

Authors

  • Attyaf Jameel Thamir Al-Tamimi

Abstract

          This study was conducted to fingerprint of seven squash genotypes using RAPD –PCR and SDS electrophoresis techniques . RAPD profile generated high level of polymorphism.                                                                                                                                                                                                                       OPA-03 and OPC-19 primers showed high value for number of main, amplified and unique bands and success in giving a distinct fingerprint for all genotypes while the primer OPT-08 gave fingerprint for one genotype .The highest value for primer efficiency and discriminatory value were produced by the primers OPD-13 and OPN-06.

         Phylogenetic tree of squash grouped the seven genotypes in to two major groups . First small main group include one genotype while the other  large main group include six genotypes which can divided in to two subgroups. The first small subgroup include one genotype while the other large subgroup include five genotypes.

         Highest genetic distance was 0.84 observed between genotypes Delicata(5) and Straight neck (7). While the lowest genetic distance 0.12 observed between Elite(2) and Ford hook (3) genotypes.

         SDS electrophoresis pattern of total seed protein showed low level of  polymorphism due to producing four monomorphic bands from six main bands . Protein profile gave fingerprint to one genotype . RAPD-PCR technique showed high polymorphism 63.6 compared with low polymorphism 33.3which produced by SDS electrophoresis technique. Thus RAPD markers provides an excellent tool for future studies of squash genotypes fingerprinting using both OPA-03 and OPC-19 primers .       

Key words: Cucurbita  pepo, Random Amplified Polymorphic DNA (RAPD),Cluster                                                                                   analysis , , fingerprint , protein profile .     

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Published

2014-04-13

How to Cite

Thamir Al-Tamimi, A. J. (2014). Genetic Fingerprint of Some Cucurbita pepo (Summer squash) Genotypes Using Molecular and Biochemical Techniques. Al-Kufa University Journal for Biology, 6(1). Retrieved from https://journal.uokufa.edu.iq/index.php/ajb/article/view/8174

Issue

Vol. 6 No. 1 (2014): Magazine of Al-kufa University for Biology.

Section

Articles

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Copyright (c) 2014 Attyaf Jameel Thamir Al-Tamimi

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