Isolation and identification of Helicobacter pylori by PCR from drinking water supplies in Erbil city
DOI:
https://doi.org/10.36320/ajb/v10.i1.8200Keywords:
H. pylori, pcr, Ure A and ure C geneAbstract
Out of the 200 samples of drinking water, which were collected from different source in Erbil city, from the period November 2014 until April 2015 . In this study, 36 isolates of Helicobacter pylori were isolated from samples as well as from biofilms, this study directed for the isolation of Helicobacter pylori by culturing in different media and identified by biochemical tests and PCR technique, in order to evaluate the potential role of drinking water in transmission of H. pylori. Water samples cultured on Brain heart infusion agar with supplement, Colombia agar base with supplement and Brucella agar; the results showed that The total number and percentage of the isolates of H. pylori from all tested water samples were 36 (18%), and these distributed as follow: 12 (24%), 11(22%), 8(16%) and 5(10%) from biofilms, reservoir, groundwater and tap water respectively. All H. pylori isolates were identified by using cultural , morphological , biochemical characteristics, from all(36) of isolates:30(83%) gave positive reaction for urease indicates that the isolates had the ability to produce the urease as well as 24(67%) of the total isolates; oxidase and catalase gave positive reaction while 17(61%) of the total isolates are motile. 35(97%) of the total samples confirmed by using primers for identification of H. pylori based on 16SrRNA this was the first report on using 16SrRNA amplification and confirmation of H. pylori isolates from water samples in Iraq. The ureA genotype was expected to be present in nearly all 34(94%) Helicobacter positive isolates. The PCR assay employed in this work specifically targets a region of the ureC (glmM) gene which has been shown to be unique and essential for the growth of H. pylori and the results appeared that 28(78%) of the total isolates have this gene This study was succeeding to detect the ureA and C gene in most of the isolates which was already confirmed by16SrRNA.Downloads
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