The protective effect of probiotics ( Lactobacillus acidophilus against urinary tract infections caused by Proteus mirabilis in vitro

Abstract

Experiment studied the protective effect of probiotics bacteria Lactobacillus acidophilus against urinary tract infections in laboratory mice caused by Proteus mirabilis, by the preparation of  heat-killed  bacteriaLactobacillusacidophilus.    13 animals were divided into three groups, the first group contained  five animals not treated, the second group contained five animals treated with the   Lactobacillus acidophilus, in addition to the control group, which contained tow animals . The second group was injected over a five-day by (o.5) ml of stuck cells of heat killed L.acidophilus  before  challenge  with 0.2 ml of the Proteus mirabilis (10⁸/cells per ml). The results showed that there was no evolution of infection with the   Proteus mirabilis in the second group of animals, while injury has evolved significantly in the  animals of first group non-treated with probiotic bacteria through not observing of the growth of the  Proteus mirabilis on the culture media for blood and tissue samples taken from animals treated. A differential count of white blood cells results showed, an increase in the number of cells, particularly neutrophils  in all the results examined. On the other hand study showed the results of the tissue sections of animals treated and non-treated for histological changes significantly for the untreated animals with the probiotics   in compare with animals  were simple changes.