Isolation and identification of bacteria from patients with cholecystits and cholelithiasis undergoing cholecystectomy

Abstract

Fourty patients were included in this study, which was extending from April 2008 till October 2008. The patients were attending the Al-Sader Teaching Hospital in Al-Najaf province.  They were all undergoing clinically and ultrasound examination. 16 of them were with cholecystits and 24 were with cholelithiasis. 11 were males and 29 were females. The age range was 15-65 years. All patients were undergoing cholecystectomy. Bile fluid and pieces of gallbladder were collected from each patient and cultured on routine bacteriological media. Identification of bacterial growth was based on standard bacteriological criteria. The results revealed thatall patients were culture positive. Escherichia coil represent the most common isolates (75%) in cholecystits, and (79.17%) in cholelithiasis, while β-hemolytic streptococci were found in (12.5%) of cholecystits cases and (8.33%) of cholelithiasis cases. Klebsiella spp. were found in (6.25%) of cholecystits and (12.5%) of cholelithiasis cases. Proteus spp. were isolated from (6.25%) of patients with cholecystits. It can be concluded that the isolation rate of Gram's negative bacteria were higher than that of Gram's positive bacteria from patients with cholecystitis and cholelithiasis undergoing cholecystectomy.