Detection of Prostate specific antigen and LDH in Toxoplsama gondii infected prostate Tissue using immunohistochemical and molecular techniques
DOI:
https://doi.org/10.36320/ajb/v18.i1.22842Keywords:
prostatitis, IHC, RT-qPCR, PSA, LDH, Najaf, Iraq.Abstract
Background: Toxoplasma gondii is a highly prevalent single-celled protozoan parasite that is estimated to infect billions of people worldwide, making it one of the most common human pathogens. Although humans are considered intermediate hosts,meaning the parasite cannot complete its full sexual life cycle, humans acquire the infection primarily through.
Mehods: A total of 100 paraffin embedded blocks containing fine needle aspiration tissues from patients suspected with prostatitis as per the histopathological report. A total of 50 paraffin embedded blocks containing healthy prostate tissues as confirmed by the histopathologist were used as control tissues. All samples underwent routine histological examination using hematoxylin and eosin (H&E) staining to assess overall tissue structure. Immunohistochemical (IHC) assays were performed to detect Prostate specific antigens and lactate dehydrogenase . Quantitative polymerase chain reaction (qRT-PCR) was used to assess the gene expression levels of PSA and LDH gene.
Results: the results of this study showed weak Immunohistochemical staining for Anti prostate specific antigens and Positive Immunohistochemical staining for anti LDH in T. gondii infected prostate tissue and elevated molecular gene expression of the PSA and LDH gene compared to control tissues.
Conclusion: the aim of this study is evaluate the pathogenic effect of T. gondii on prostate tissues in patients clinicaly diagnoses with Prostatitis.
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