Protective Role of Pomegranate Peel (Punica granatum L) against Thioacetamide-Induced cirrhosis in a Rat Model
DOI:
https://doi.org/10.36320/ajb/v9.i2.7592Keywords:
TAA Thioacetamide , PP pomegranate peel , DDR2 Discoidin Domain ReceptorAbstract
This study was designed to determine the effects of pomegranate peel on various antioxidiants measurements in liver cirrhosis of rats induced by thioacetamide.Fifty-seven male rats aged 13–15 weeks were randomly divided into 15 categories containing 5 rats per category, and were treated with either TAA, PP, or PP plus TAA or Selenium plus TAA or administered with 0.9% physiological saline solution as a control. The hepatoprotective effect of herbs was evaluated by measuring levels of antioxidiant marker enzymes such as Glutathione Peroxidase (GSH-Px) and Superoxide Dismutase (SOD) and measurement Malondialdehyde (MDA). The histological studies were also carried out to support the above parameters by using special stain Haemotoxlin & eosin and using immunofluorescent staining to examine the distribution of DDR2 in the liver. The study found that the supplementation of pomegranate peel significantly (p< 0.05) reduced the damaging effects on the liver by TAA. GSH, SOD increased in the treatment group, but serum MDA level was declined due to the hepatic injury induced. A comparative histopathological research of the liver between different groups demonstrated that pomegranate peel, drive to normal liver architecture suggesting they may use as a hepatoprotective alternative medication
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B. E. Myagmar, E. Shinno, T. Ichiba, and Y. Aniya (2004) .Antioxidant activity of medicinal herb rhodococcum vitis-idaea on galactosamine-induced liver injury in rats.Phytomedicine, 11(5) , 416–423,.
M. A. Alshawsh, M. A. Abdulla, S. Ismail, and Z. A. Amin,( 2010 ).Hepatoprotective effects of orthosiphon stamineus extract on thioacetamide-induced liver cirrhosis in rats. Evidence-Based (Boesenbergia rotunda, Boesenbergia pulchella var attenuate and Boesenbergia armeniaca),” Journal of Medicinal Plant Research, 4 (1),27–32.
Raison, C. L., Demetrashvili, M., Capuron, L., & Miller, A. H. (2005). Neuropsychiatric adverse effects of interferon-α. CNS drugs, 19(2), 105-123.
Yang, H., Sung, S. H., & Kim, Y. C. (2005). Two New Hepatoprotective Stilbene Glycosides from Acer m ono Leaves. Journal of natural products, 68(1), 101-103.
Wang, N., Li, P., Wang, Y., Peng, W., Wu, Z., Tan, S., ... & Su, W. (2008). Hepatoprotective effect of Hypericum japonicum extract and its fractions. Journal of Ethnopharmacology, 116(1), 1-6.
El-Abhar, H. S., Hammad, L. N., & Gawad, H. S. A. (2008). Modulating effect of ginger extract on rats with ulcerative colitis. Journal of ethnopharmacology, 118(3), 367-372.
Harborne, J. B. (1984). Methods of plant analysis. In Phytochemical methods(pp. 1-36). Springer Netherlands.
Chattopadhyay, R. (2003). Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract: Part II. Journal of ethnopharmacology, 89(2), 217-219.
Burtis, C. A., Ashwood, E. R., & Bruns, D. E. (2012). Tietz textbook of clinical chemistry and molecular diagnostics. Elsevier Health Sciences.
Armstrong, D.; Browne, R. (1994) Free Rad. Diag. Med. 366; 43-58.
.Liu, J., Liu, Y., Parkinson, A., & Klaassen, C. D. (1995). Effect of oleanolic acid on hepatic toxicant-activating and detoxifying systems in mice. Journal of Pharmacology and Experimental Therapeutics, 275(2), 768-774.
Poli, G. (2000). Pathogenesis of liver fibrosis: role of oxidative stress.Molecular aspects of medicine, 21(3), 49-98.
Rice-Evans, C. (1995). Plant polyphenols: free radical scavengers or chain-breaking antioxidants?. In Biochemical Society Symposia (Vol. 61, pp. 103-116). Portland Press Limited.
Ajith, T. A., Hema, U., & Aswathy, M. S. (2007). Zingiber officinale Roscoe prevents acetaminophen-induced acute hepatotoxicity by enhancing hepatic antioxidant status. Food and chemical toxicology, 45(11), 2267-2272.
.Okuyama, H., Nakamura, H., Shimahara, Y., Araya, S., Kawada, N., Yamaoka, Y., & Yodoi, Y. (2003). Overexpression of thioredoxin prevents acute hepatitis caused by thioacetamide or lipopolysaccharide in mice. Hepatology, 37(5), 1015-1025.
Staňková, P., Kučera, O., Lotková, H., Roušar, T., Endlicher, R., & Červinková, Z. (2010). The toxic effect of thioacetamide on rat liver in vitro. Toxicology in vitro, 24(8), 2097-2103.
Sanz, N., Dı́ez-Fernández, C., Andrés, D., & Cascales, M. (2002). Hepatotoxicity and aging: endogenous antioxidant systems in hepatocytes from 2-, 6-, 12-, 18-and 30-month-old rats following a necrogenic dose of thioacetamide. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1587(1), 12-20.
Díez-Fernández, C., Sanz, N., & Cascales, M. (1996). Changes in glucose-6-phosphate dehydrogenase and malic enzyme gene expression in acute hepatic injury induced by thioacetamide. Biochemical pharmacology, 51(9), 1159-1163.
Dickinson, D. A., & Forman, H. J. (2002). Cellular glutathione and thiols metabolism. Biochemical pharmacology, 64(5), 1019-1026.
Han, D., Canali, R., Rettori, D., & Kaplowitz, N. (2003). Effect of glutathione depletion on sites and topology of superoxide and hydrogen peroxide production in mitochondria. Molecular pharmacology, 64(5), 1136-1144.
Okuyama, H., Nakamura, H., Shimahara, Y., Araya, S., Kawada, N., Yamaoka, Y., & Yodoi, Y. (2003). Overexpression of thioredoxin prevents acute hepatitis caused by thioacetamide or lipopolysaccharide in mice. Hepatology, 37(5), 1015-1025.
Cheng Haung W, Yannjan C,Tsung Hsing L, Yi-Shen C, Bruno J, Kuo Sheng H, Chengnan H, Jong Kalg L.(2004).Protective effect of MDL 28170 against thioacetamide induced acute liver failure in mice". J. Biomed Sci ,11, 571 - 578.
Abul, H., Mathew, T. C., Dashti, H. M., & AL‐BADER, A. (2002). Level of Superoxide Dismutase, Glutathione Peroxidase and Uric Acid in Thioacetamide‐Induced Cirrhotic Rats. Anatomia, histologia, embryologia,31(2), 66-71.
Akbay, A., Ĉinar, K., Uzunalimoglu, Ö., Eranil, S., Yurdaydin, C., Bozkaya, H., & Bozdayi, M. (1999). Serum cytotoxin and oxidant stress markers in N-acetylcysteine treated thioacetamide hepatotoxicity of rats. Human & experimental toxicology, 18(11), 669-676.
Ammon, H. P., Grimm, A., Lutz, S., Wagner-Teschner, D., Handel, M., & Hagenloh, I. (1980). Islet glutathione and insulin release. Diabetes, 29(10), 830-834.
Frederiks W, Bosh K D E, Jong J , Noorden C.(2003). Posttranslational regulation of glucose- 6- phosphate dehydrogenase activity in (pre) neoplastic lesions in rat liver . J. Hist. Cyto., 51, 105 – 112.
Singh, R. P., Chidambara Murthy, K. N., & Jayaprakasha, G. K. (2002). Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. Journal of agricultural and food chemistry, 50(1), 81-86.
Lansky, E. P., & Newman, R. A. (2007). Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of Ethnopharmacology, 109, 177–206.
Bishayee, A., Bhatia, D., Thoppil, R. J., Darvesh, A. S., Nevo, E., & Lansky, E. P. (2011). Pomegranate-mediated chemoprevention of experimental hepatocarcinogenesis involves Nrf2-regulated antioxidant mechanisms. Carcinogenesis, 32(6), 888–896.
Moskaug, J., Carlsen, H., Myhrstad, M. C., & Blomhoff, R. (2005). Polyphenols and glutathione synthesis regulation. The American Journal of Clinical Nutrition, 81, 277S–283S.
Route, S., & Banerjee, R. (2007). Free radical scavenging, anti-glycation and tyrosinase inhibition properties of a polysaccharide fraction isolated from the rind from Punica granatum. Bioresource Technology, 98, 3159–3163.
Ajaikumar, K. B., Asheef, M., Babu, B. H., & Padikkala, J. (2005). The inhibition of gastric mucosal injury by Punicagranatum L.(pomegranate) methanolic extract. Journal of ethnopharmacology, 96(1), 171-176.
Osman MM, Ahmed S. Mahfouz S.(2011) Biochemical studies on the Hepatoprotective effects of Pomegranate and Guava ethanol extracts. New York Science J.,4, 27-41.
Toklu, H. Z., Sehirli, O., Sener, G., Dumlu, M. U., Ercan, F., Gedik, N., & Gökmen, V. (2007). Pomegranate peel extract prevents liver fibrosis in biliary‐obstructed rats. Journal of Pharmacy and Pharmacology, 59(9), 1287-1295.
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Copyright (c) 2017 Adnan W. Al-Bideri, Alaauddin S.M.Al-sallami, Shaymaa H.Alsaaedi
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