Antibacterial Activity of Essential oils Isolated from Eucalyptus globulus Labill and Eugnia caryophyllata Thunbery (Family Myrtaceae): A comparative study
DOI:
https://doi.org/10.36320/ajb/v10.i2.8128Keywords:
Essential oils , Eugnia caryophyllata , Eucalyptus globulusAbstract
The essential oils of Eucalyptus globulus Labill and Eugnia caryophyllata Thunbery analyzed by Thin Layer Chromatography TLC. The results showed that TLC plates separated five compounds from E. globulus oils and four compounds from E. caryophyllata oils with different Rf values. The obtained essential oils from leaves E. globulus and flower buds E. caryophyllata were tested on Escherichia coli and Staphylococcus aureus, by using agar well diffusion method, to determine its antibacterial activities. The strongest effected was noted in E. globulus oils against S. aureus and E.coli with diameter of zone of inhibition (25 mm and 23 mm respectively). While the E. caryophllata oils showed the least effect on S. aureus and E. coli in compare with E. globulus oils with diameter of zone of inhibition (20 mm and 18 mm respectively). We concluded that the essential oils obtained from the leaves of E. globulus offer significant antibacterial activity toward E.coli and S. aureus.
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Aggarwal, K K; Khanuja, S P S; Ahmad, A; Kumar, T R S; Gupta, V K; Kumar, S. (2002). Antimicrobial activity profiles of the two enantiomers of limonene and carvone isolated from the oils of Mentha spicata and Anethum sowa. Flavour and Fragrance Journal, 17(1): 59-63.
Angela, E; Sadlon, N D; Davis, W; Lamson, M S. (2010). Immune-modifying and antimicrobial effect of Eucalyptus oil and simple inhalation devices, Altern med Rev, 15: 33-47. 3. Ayoola, G A; Lawore, F M; Adelowotan, T; Aibinu, I E;
Adenipekun, E et al (2008). Chemical analysis and antimicrobial activity of essential oil of Syzygium oromaticum (Clove). Afr J Microbiol Res, 2: 162- 166.
Bhagat, M; Gupta, S; Jamwal, V S; Sharma, S; Kattal, M; Dawa, S; Devi, R; Bindu, K. (2016). Comparative study on chemical profiling and antimicrobial properties of essential oils from different parts of Eucalyptus lanceolatus. Indian Journal of Traditional Knowledge, 15 (3): 425-432.
Briozzo, J; Nunez, L; Chirife, J; Herszage, L; Aquino, DM. (1989). Antimicrobial activity of clove oil dispersed in a concentrated sugar solution. J. Appl. Bacteriol., 66(1): 69-75.
Brooker, M I H; Kleinig, D A. (2004). Field Guide to Eucalypts, volume 3:Northern Australia. Blooming Books, Victoria, Australia.
Cai, L; Wu, CD. (1996). Compounds from Syzygium oromanticum possessing growth inhibitory activity against oral pathogens. J Nat Prod, 59 (10): 987- 90.
Chaieb, K; Zmantar, T; Kouri, R; Hajlaoui, H; Mahdouani, K; Abdelly, C. (2007). Antioxidant properties of essential oil of Eugenia caryophyllata and its antifungal activity against a large number of clinical Candida species. Mycosis, 50 (5): 403-6.
Denyer, S P; Hugo, W B. (1991). Biocide –induced damage to the bacterial cytoplasmic membrane. In: Denyer S P; Hugo, W B. (Eds). Mechanism of Action of Chemical Biocide. Blackwell Scientific Publication, Oxford, pp. 171-188.
Endo, T; Ogihima, K; Tanaka, H; Oshima, S. (1972). Studies on the anaesthetic effect of eugenol in some fresh water fishes. Bulletin of Japanese Society of Fish Science, 38: 761-7.
Feyza, O; Belma, A; Sahlan, O; Senol, A. (2009). Essential oil composition, antimicrobial and antioxidant activities of Satureja cuneifolia Ten. Food Chem, 112: 874-879.
Figueiredo, A C; Barroro, J G; Pedro, L G; Salgueiro, L; Miguel, M G; Faleiro, M L. (2008). Portuguese Thymbra and Thymus Species Volatiles: Chemical Composition and Biological Activities. Curr Pharm Des, 14: 3120- 40.
Fredj, M B H; Marzouk, B; Chraief, I; Boukef, K; Marzouk, K. (2007). Analysis of Tunisian Ruta graveolens L. oil from Jemmel. J. Food Agric. Environ, 5(1): 52-55.
Harborne, J B. (1998). Essential oils. In: Phytochemical Methods: A guide to modern techniques in plant analysis (3rd edition). Chapman and Hall Co, New York. pp 110 – 124.
Hema, R; Kumaravel, S; Sivasubramanian C. (2010). GC-MS study on the potentials of Syzygium oromaticum. Researcher, (12): 1-4.
Henriette, K. (2002). Oleum Eucalypti, B.P. oil of Eucalyptus. The British Pharmaceutical Codex. http://www.henriettes-herb.com/eclectic/bpc 1911/eucalyptus_Oleu.html, accessed on 8 August, 2013.
Ibrahim, M S; AL-Zubaidi, A L; Adnan, S A; Jessim, A I; Jasim, I A. (2015). Antioxidant activity of purified Eugenol compound in some dairy products. International Journal of Advanced Research, 3(4): 186-195.
Isaacs, G. (1983). Permanent local anaesthesia and anhydrosis after clove oil spillage. Lancet, 321(8329): 882-3.
Lopez, P; Sanchez, C; Batlle, R; Nerin, C. (2005). Solid- and vapor-phase antimicrobial activities of six essential oils: susceptibility of selected food borne bacterial and fungal strains. Journal of Agriculture and Food Chemistrym, 53: 46-6939.
Magiatis, P; Skaltsounis, A L; Chinou, I; Haroutounian, S A. (2002). Chemical composition and in-vitro antimicrobial activity of the essential oils of three Greek Achillea species. Z. Naturforsch. 59: 287-290.
Miguel, M G; Duarte, J; Figueiredo, A C; Barroso, J G; Pedo, L G. (2005). Thymus carnosus Boiss: Effect of harvesting period, collection site and type of plant material on essential oil composition. Journal of Essential oil Research, 17 (4): 422-426.
Musyimi, D M; Ogur, J A. (2008). Comparative assessment of antifungal activity of extracts from Eucalyptus globules and Eucalyptus citriodora. Research Journal of Phytochemistry, 2:35-43.
Ody, P. (1994). The Complete Medicinal Herbal. Dorling Kindersley, London: 61.
Palanna, R M. (1996). Eucalyptus in India .In: Report submitted to the regional expert consultation on eucalyptus. RAP Publication, Bangladesh, vol. 2: 1-280.
Perini, S; Piccoli, R H; Nunes, C A; Bruhn, F R P; Custodio, D A C; Costa, G M. (2014). Antimicrobial activity of essential oils against pathogens isolated from bovine mastitis. J Nat Prod plant Resour, 4(2): 6-15.
Pozzo, M D; Viegas, J; Santuario, D; Rossatto, L; Soares, I H; Alves, S; Costa, M. (2011). Activity of essential oil from spices against Staphylococcus spp. Isolated from bovine mastitis. Arquivo Brasileiro de Medicina Veterinaria Zootecnia. 63: 1229-1232.
Ross, L G; Ross, B. (1999). Anaesthetic and Sedative Techniques for Aquatic Animals. 2nd ed. Blackwell Science Ltd: Oxford , P. 159.
Rota, C; Carraminana, J J; Burillo, J; Herrera, A. (2004). In-vitro antimicrobial activity of essential oils from aromatic plants against selected foodborne pathogens. J. Food Prot. 67: 1252 – 1256.
Sikkema, J; de Bont JAM; Poolman, B. (1994). Interaction of cyclic hydrocarbons with biological membranes. J. Biology. Chem., 269 (11): 8022- 28.
Singh, J; Baghotia, A; Goel, S P. (2012). Eugenia caryophyllata Thunberg (Family Myrtaceae): A review. International Journal of Research in Pharmaceutical and Biomedical Science, 3(4): 1469-75.
Taylor, P W; Roberts, S D. (1999). Clove oil: An alternative anaesthetic for aquaculture. North American Journal of Aquaculture, 61: 150-155.
raore, N; Bouare, S; Sidibe, L; Somboro, A A; Fofana, B; Tangara, O et al (2014). Antimicrobial activity of essential oils of Eucalyptus camaldulensis from Mali. Asian Journal of Plant Science and Research, 4(4): 69-73.
Tserennadmid, R; Tako, M; Galgoczy, L; Papp, T; Pesti, M; Vagvolgyi, C; Almassy, K; Krisch, J. (2011). Anti-yeast activities of some essential oils in growth medium, fruit, juices and milk. Int J Food Microbiol, 144(3): 480- 486.
Viljoen, A; Vuuren, S V; Ernst, E; Klepser, M; Demirci, B; Baser, H; van Wyk, B E V. (2003). Osmitopsis asteriscoides (Asteraceae). The antimicrobial activity and essential oil composition of a Cape-Dutch remedy. Journal of Enthophopharmacol., 88(2-3): 137-43.
Yang, S J; Kang, P; Min, S S; Lee, J-M; Kim, H-K; Seol, G H. (2013). Effect of Eucalyptus oil Inhalation on pain and inflammatory responses after total knee replacement: A randomized clinical trial. Evid Based Complemet Alternative Med, doi: 10.1155/ 2013/ 502727.
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