Comparative study for some natural products between two species of Anoectochilus genus tissue culture and wild
DOI:
https://doi.org/10.36320/ajb/v8.i1.8029Keywords:
A.roxiburghii , medicinal plant, traditional uses, chemical constituents, Anoectochilus formansus.Abstract
Anoectochilus genus is epiphytic Orchid used as traditional medicine. Chemical components and pharmacology have been studied in recent 15 years. Medicinal orchid, in general, is not subjected to detailed pharmacological studies. The aim of this study was to estimate and compare the concentration of bioactive compounds between wild and in vitro propagated Anoectochilus roxburghii and Anoectochilus formosanus. A wide range of chemical compounds are presented including flavonoids, steroids and oil violate which have been isolated recently from these species. Extract and metabolite of these plants, particularly from whole plant , possess useful pharmacological activities .A comprehensive account of chemical constituents and biological activities is presented and a critical appraisal of the ethno pharmacological issues . these species orchid have been empirically used for treatment of different diseases . The results show that the flavonoid, steroid and essential oil contents in three fields were (tissue culture ,after three months and after 6 months in wild) for each species which regarding tissue culture (flavonoid ,steroids and oil essential ) In A.roxiburghii, the flavonoid contents were (2%,0.9%,6.5%),steroids contents(0.003%,0.007%.0.01%) and the oil essential contents (0.05%,0.3%,0.18%) where as for A.formosanus flavenoid contents (6%, 1.05%,6%) steroids contents(0.2,0.005,0.2)% and the oil essential contents (0.1,0.18,0.17)%
Downloads
References
Tseng, C.C., Shang, H.F., Wang, L.F., Su, B., Hsu, C.C., Kao, H.Y., Cheng, K.T., 2006. Antitumor and immune stimulating effects of Anoectochilus formosanus Hayata. Phytomedicine 13, 366–370. DOI: https://doi.org/10.1016/j.phymed.2004.01.016
Ket, N.V., Hahn, E.J., Park, S.Y., Chakrabarty, D., Paek, K.Y., 2004. Micropropagation of an endangered orchid Anoectochilus formosanus. Biol. Plant 48, 339–344. DOI: https://doi.org/10.1023/B:BIOP.0000041084.77832.11
Du, X.M., Irino, N., Furusho, N., Hayashi, J., Shoyama, Y., 2008. Pharmacologically active compounds in the Anoectochilus and Goodyera species. J. Nat. Med.Tokyo 62, 132–148. DOI: https://doi.org/10.1007/s11418-007-0169-0
Cui, S.C., Yu, J., Zhang, X.H., Cheng, M.Z., Yang, L.W., Xu, J.Y., 2013. Antihyperglycemic and antioxidant activity of water extract from Anoectochilusroxburghii in experimental diabetes. Exp. Toxicol. Pathol. 65, 485–488. DOI: https://doi.org/10.1016/j.etp.2012.02.003
Shao, Q.S., Deng, Y.M., Liu, H.B., Zhang, A.L., Huang, Y.Q., Xu, G.Z., Li, M.Y., 2014a. Essential oils extraction from Anoectochilus roxburghii using supercritical carbon dioxide and their antioxidant activity. Ind. Crops Prod. 60, 104–112. DOI: https://doi.org/10.1016/j.indcrop.2014.06.009
Shao, Q.S., Zhou, A.C., Hu, R.H., Zhang, Y.Y., Liu, T.M., Li, M.Y., 2014b. Influence of seedling grade on plant growth, yield and quality of Anoectochilus roxburghii. China J. Chin. Mater. Med. 39, 785–789.
He, C.N., Wang, C.L., Guo, S.X., Xiao, P.G., 2004. Research progress on chemical constituents and pharmacological activities of Anoectochilus. Chin. Pharm. J. 39, 81–84.
Agricultural Science Paper, 2013.Dynamic Change of Main Active Ingredients in A. roxburghii (Wall) Lindl and A.formosanus Hayata in Different Growth Stages
Que, W. C. (2012). The Extraction Of Volatile Oil In Anoectochilus And Its Inhibition Effect In Lung Cancer Cell NCI-H446.
Huang L Y, Chen T W, Ye Z, et al. Use of liquid chromatographyatmosphericpressure chemical ionization-ion trap mass spectrometry for identification of oleanolic acid and ursolic acid in Anoectochilus roxburghii (wall.) Lindl. J Mass Spectrom, 2007, 42: 910–917 DOI: https://doi.org/10.1002/jms.1228
Du, X. M., Sun, N. Y., Irino, N., Shoyama, Y., Glycosidic Constituents from in Vitro Anoectochilus formosanus Chem.Pharmaceut. Bull. 2000, 48, 1803–1809 DOI: https://doi.org/10.1248/cpb.48.1803
Chang-Chai Ng,et al.(2011),lactic acid bacterial fermentation on the production of functional antioxidant herbal Anoectochilus formosanus Hayata. National Taiwan University .No3,289-293 DOI: https://doi.org/10.1016/j.jbiosc.2010.11.011
Yoon, Y.J., Murthy, H.N., Hahn, E.J., Paek, K.Y., 2007. Biomass production of Anoectochilus formosanus Hayata in a bioreactor system. J. Plant Biol. 50,573–576. DOI: https://doi.org/10.1007/BF03030711
Wang, Y.J., Meng, Z.X., Yu, X.M., Wang, C.L., Guo, S.X., 2009. Screening of endophytic fungi promoting growth and development of Anoectochilus roxburghii. Chin. Pharm. J. 44, 976–979.
Li, B., Tang, M.J., Tang, K., Zhao, L.F., Guo, S.X., 2012. Screening for differentially expressed genes in Anoectochilus roxburghii (Orchidaceae) during symbiosis with the mycorrhizal fungus Epulorhiza sp. Sci. China Life Sci. 55, 164–171. DOI: https://doi.org/10.1007/s11427-012-4284-0
Kong, X.H., 2001. Preliminary study on natural resources of Anoectochilus roxburghii. Chin. Tradit. Herb. Drugs 32, 155–157
Ailian Zhang, Hongzhen Wang, Qingsong Shao , Mengjie Xu ,Wangshu Zhang, Mingyan Li ,2015. Large scale in vitro propagation of Anoectochilus roxburghii for commercial application: Pharmaceutically important and ornamental plant. Zhejiang University.70. 158–162. DOI: https://doi.org/10.1016/j.indcrop.2015.03.032
Fan, Z.N., Xiao, H.S., Fan, X.H., Wu, W.S. (1997). Study on tissue culture of Anoectochilus roburghii. Journal Fujian Normal University (Nat SciEd) 13: 82-87.
Guo S X, Chen X M, Yu X M, et al. Investigation on the isolation of mycorrhizal fungi from Anoectochilus roxburghii and its biological activity. Chin Pharm J, 2000, 35: 443–445
Pant B, Raskoti BB (2013). Medicinal Orchids of Nepal. Himalayan Map House, Pvt. Ltd. (Publisher)
Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bio assays with tobacco tissue culture. Physiol. Plant 15, 473–497. DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Ziv M (1995) Developmental and structural patterns of in vitro plants. In: Woong-Young S, Sant SB (eds) Morphogenesis in Plant Tissue Culture 9, 234-253
Ziv M (1986) In vitro hardening and acclimatization of tissue culture plants. In: Withers LA, Alderson PG (eds) Plant Tissue Culture and its Agricultural Applications, Butterworths, London, the UK, pp 187-196 DOI: https://doi.org/10.1016/B978-0-407-00921-9.50024-5
Ziv M, Schwarts A, Fleminger D (1987) Malfunctioning stomata in vitreous leaves of carnation (Dianthus caryophylus) plants propagated in vitro; implications for hardening. PlantScience 52, 127-134 DOI: https://doi.org/10.1016/0168-9452(87)90114-2
Chun-Nian He, Chun-Lan Wang,Shun-Xing Guo*,Jun-Shan Yang and Pei-Gen Xiao ,2006.A Novel Flavonoid Glucoside from Anoectochilus roxburghii (Wall.) Lindl.
Yonghui Zhang, Jinyan Cai, Hanli Ruan,Huifang Pi, Jizhou Wu.2007. Antihyperglycemic activity of kinsenoside, a high yielding constituent from Anoectochilus roxburghii in streptozotocin diabetic rats ,Journal of Ethnopharmacology, Pages 141–145 DOI: https://doi.org/10.1016/j.jep.2007.05.022
Flora Reipublicae Popularis Sinicae .2000,editorial Committee of Flora of China, Chinese Academy of Sciences.. Beijing: Science Press; 204
Martha. R., Gutiérrez. P.,Fijo. P. (2009). Orchids: A review of uses in traditional medicine, its phytochemistry and pharmacology 16, Col. Torres Lindavistacp 07708, Mexico D.F, Mexico
S .L. Zhu,2011. Analysis On Active Constituents Of Anoectochilu Roxburghii Pharmacological journal
Han .M.H; Yang. X.W; Jin .Y.P ,2008.Novel triterpenoid acyl esters and alkaloids from Anoectochilus roxburghii. .Phytochem Anal. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083, People's Republic of China:438-43 (ISSN: 1099-1565) DOI: https://doi.org/10.1002/pca.1070
Li YanDong; Ye HongXia; Chen LiPing 2013. Study on tissue culture and rapid propagation techniques of Anoectochilus roxburghi Acta Agriculturae Jiangxi Vol. 25 No. 4 pp. 52-54, 62 ISSN1001-8581.
RAN Cai-hong .2009, Anoectochilus formosanus Culture Seedling Rooting Culture (Fujian Forestry Science and Technology Test Center,Zhangzhou 363600,Fujian,China)
Ernst, R.: Effects of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orchidaceae). - Plant Cell Tissue Organ Cult. 39: 273-275, 1994. DOI: https://doi.org/10.1007/BF00035982
Chen, Y.Q., Piluek, C.: Effects of thidiazuron and N6- benzylaminopurine on shoot regeneration of Phalaenopsis. - Plant Growth Regul. 16: 99-101, 1995. DOI: https://doi.org/10.1007/BF00040513
Nayak, N.R., Patnaik, S., Rath, S.P.: Direct shoot regeneration from foliar explants of an epiphytic orchid Acampe praemorsa (Roxb.) Blatter and McCann. - Plant Cell Rep. 16: 583-586, 1997. DOI: https://doi.org/10.1007/s002990050283
Zhao, D.L., Guo, G.Q., Wang, X.Y., Zheng, G.C.: 2003/04 In vitromicropropagation of a medicinal plant species Sophora flavescens. - Biol. Plant. 47: 117-120. DOI: https://doi.org/10.1023/A:1027397302296
Zhang FS, Lv YL, Dong HL, Guo SX. Analysis of genetic stability through intersimple sequence repeats molecular markers in micropropagated plantlets of Anoectochilus formosanus HAYATA, a medicinal plant. Biol Pharm Bull. 2010;33(3):384–388. doi: 10.1248/bpb.33.384. DOI: https://doi.org/10.1248/bpb.33.384
Huetteman, C.A., Preece, J.E.: Thidiazuron: a potent cytokinin for woody plant tissue culture. - Plant Cell Tissue Organ Cult. 33: 105-119, 1993. DOI: https://doi.org/10.1007/BF01983223
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2019 Nada Mohammed Reda Refish, Chunhua Fu
This work is licensed under a Creative Commons Attribution 4.0 International License.
which allows users to copy, create extracts, abstracts, and new works from the Article, alter and revise the Article, and make commercial use of the Article (including reuse and/or resale of the Article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made and the licensor is not represented as endorsing the use made of the work.
