Molecular identification and Virulence factors of Pseudomonas aeruginosa isolated from operation hall
DOI:
https://doi.org/10.36320/ajb/v13.i2.11758Keywords:
Virulence factors, P.aeruginosa, opportunistic pathogenAbstract
This study aimed to determine the P.aeruginosa that causes contamination to operation hall in hospitals by detecting of pathogenisity markers ..Moreover, the virulence factors of predominant species were detected phenotypically by using routine techniques, that responsible for pathogenicity.
Fifteen samples were collected from different sites of operation hall during two months 2022 in AL-Hussein hospital/ karbala City. The identification of P.aeruginosa isolates depended on colonial morphology, microscopic examination and biochemical tests as a primary identification. The final identification was confirm by PCR technique from different sites .The results obtained by the PCR tests were twenty two isolates of P.aeruginosa were detected , which distributed into :(9) earth, (8) wall, and (10) beds.
The study investigated the virulence factors of P.aeruginosa, which had the ability to produce capsule, biofilm , adhesion ,protease, bacteriocin ,haemolysin and β-lactamase and gelatinase .
The results revealed variation in the resistance of bacteria to some antibiotics,..P.aeruginosa exhibited high resistance (96%) to Cefotaxime, but absolute susceptibility to Ciproflaxacin and Gentamycin and high susceptibility to Amikacin.Ceftiaxone,and Azithromycin.
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