Dissemination of Metallo-β-Lactamase Genes in Pseudomonas aeruginosa Isolated from Najaf Hospitals
DOI:
https://doi.org/10.36320/ajb/v7.i3.8066Keywords:
Metallo-β-Lactamase, Pseudomonas aeruginosa, Efflux pumpsAbstract
Carbapenems are therapeutic choice against infections caused by Gram-negative bacilli including strains of Pseudomonas aeruginosa. Efflux pumps, porins, PBPs and β-lactamases mediate resistance to these antibiotics. The aim of this study was to determine the possibility of existence of metallo-β-lactamase (MBL) genes among P. aeruginosa isolates collected from Najaf hospitals. During the study period from February to October 2011, thirty-six carbapenem resistant P. aeruginosa isolates were collected. The susceptibility to different antibiotics was evaluated by disk diffusion method, MICs of imipenem and meropenem were also determined. PCR performed for detection of blaIMP, blaVIM, blaSPM and blaNDM genes. All isolate were multidrug resistance and 21 produced MBLs. The blaIMP, blaSPM, blaVIM and blaNDM genes were detected among 13, 8, 4 and 2 isolates, respectively. The MICs of isolates to imipenem and meropenem were 4-128 μg/mL and 4-256 μg/mL, respectively. We concluded that production of MBL enzymes presents an emerging threat of carbapenem resistance among P. aeruginosa in Najaf city. This is the first report of IMP, SPM, VIM, and NDM β-lactamase producing P. aeruginosa in IraqDownloads
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