Detection of Candida species isolated from immunocompromised patients in AL-Najaf province

Detection of Candida species isolated from immunocompromised patients in AL-Najaf province


  • Taif razzaq majjed Al-Furat Al-Awsat Technical University, Iraq
  • Mona Adel Ismail Al-Furat Al-Awsat Technical University
  • Ali Yas KhudhairAl-Amer Al-Furat Al-Awsat Technical University, Iraq



Candida species, Thalassemia, Nystatin, Amphotericin B.


Study was carried out to examine phenotyping as a means of identifying of fungal species isolated by those who suffer from Thalassemia in the province of Al-Najaf and to assess function Inhibition of Some Antifungal Agents (Nystatin and Amphotericin B). Fifty samples, including oral swabs, were taken from the AL-Zahraa Teaching Hospitel in the AL Najaf province between October and November 2021, but only 14 showed good growth.  All samples were cultured in the Microbiology Department of the Faculty of Medical Technologies at the Islamic college of Najaf, I plated them first on Sabouraud dextrose agar, and then on chromagar medium. to identify the species of Candida based on colony color. On chromagar medium, the results read as follows: C.albicans 10, C.parapsilosis 3, and C. tropicalis 1. Candida albicans' virulence factor production, including the formation of germ tubes, the formation of biofilms, and the production of phospholipase enzymes, was examined in this research. All virulence factors, such as germ tubes, biofilms, and the phospholipase enzyme, are produced by C. albicans, as evidenced by the culture findings.

This research used a disk diffusion technique to describe the effects of the antifungal drugs nystatin and amphotercin B on the yeast species Candida. Based on these results, Amphotercin B is preferable to Nystatin for treating Candida spp..


Download data is not yet available.



Aksu, T. and Ünal, Ş. (2021). Thalassemia. Trends in Pediatrics 2(1):1-7.

Carmona, E. M. and Limper, A. H. (2017). Overview of treatment approaches for fungal infections. Clin Chest Med. 38(3):393-402.

A. Zahra Jabbar Allobawi, S., Kitab Abid Zaid Alshafiee, A., Mohammed Ashour, Q., & Hadi Rahim, S. (2019). Effect of different doses of fungicide Bentazole Combl 50% S.L. on the growth of fungus Nurses Rhizactonia solani and Fusarium solani isolated from cucumber plant. Al-Kufa University Journal for Biology , 11 (1). Retrieved from https: //journal. uokufa.

Horvath,L.L.; Murray, D.R.; C.K. and Doooley, D.P.(2003). Direct isolation of Candida spp. from blood cultures on the chromogenic medium chromagar Candida .J. Clin Microbiol .41:2629-32.

Forbes, B. E.; Sahm, D. F. and Weissfeld, A.S. (2007).Bailey and scott's diagnostic Microbi -ology.12th ed. Mosby Elsevier. Texas, USA.

Branchini, M. L.; Pfaller, M. A.; Rhine-Chalberg, J. ; Frempong, T. and Isenberg, H. D. (1994). Genotypic variation and slime production among blood and catheter isolates of Candida parapsilosis. J Clin Microbiol; 32:452-6.

Deorukhkar, S. and Saini, S. (2014). “Virulence markers and antifungal susceptibility profile of Candida glabrata: an emerging pathogen ,” British Microbiol Research J. 4 (1): 35–45.

Samaranayake, L. P.; Raeside, J. M. and MacFarlane, T.W. (1984). Factors affecting the phospholipase activity of Candida species in vitro. Sabouraudia ; 22:201-7.

Sachin, C. D.; Ruchi, K. and Santosh, S. (2012). In-vitro evalution of proteinase, phospholipase and haemolysin activities of Candida species isolated from clinical specimens. Int J Med Biomed .Res. 1:153-157.

Chandrasekar, P. (2011). Management of invasive fungal infections: A role for polyenes. J. Antimicrob. Chemother.66, 457–465.

Bhavan, P.S.; Rajkumar, R.; Radhakrishnan, S.; Seenivasan, C. and Kannan, S. (2010). Culture and Identification of Candida albicans from vaginal ulcer and separation of enolase on SDS-PAGE. Inter. J. Bio. 2; (1): 84-93.

Iehab, Y. J. (2014). Isolation and Identification of Candida spp.from patients with Oral thrush in A.L.- Najaf Province and molecular study of some virulence factors. MSc, Faculty of Science/ University of Kufa.

Ali, Y. K. (2018). Genotypic characterization of fungal species isolated from patients with different cases in Al-najaf province . MSc, Faculty of Science/ University of Kufa.

Abdulkareem Hussein, K. (2016). Use of universal 18SrDNA gene and CHROMagar Candida medium for the Identification of Candida species isolated from denture wearers. Al-Kufa University Journal for Biology. Retrieved from https:// journal. Uoku

Kim, D.;Shin , W.; Lee , K.; Kim , K. and Park , J. (2002). Rapid differentiation of Candida albicans from other Candida species using it unique germ tube formation at 39 C0 . Yeast.19 : 957 – 962.

Kumar, A. ; Sharma, P. C. ; Kumar, A. and Negi, V. (2014). A study on phenotypic traits of candida species isolated from blood stream infection and thrir in vitro susceptibility to fluconazole.Al Ameen J. MED. Sci. 7(1):83-91.

Ataa, K.A. (2020). Detection of morphogenesis gene in C. albicans isolated from different clinical caces. MSc, Faculty of Science/ University of Kufa.

Emily, P. F.; Jeniel, E. N.; Trevor, R. S.; Quinn, M. M.;Aaron,D. H.; Brian, B. T.; David, R. A. and Alexander, D. J.(2011). A Recently Evolved Transcriptional Network Controls Biofilm Development in Candida albicans.

Donlan, R.M. (2002). Biofilms: microbial life on surfaces. Emerg Infect Dis.8:1–19.

Achkar, J.M. and Fries, B.C. (2010) .Candida infections of the genitourinary tract. Clin. Microbiol. 23: 253-273.

Vinitha, M. and Mamatha, B. (2011). Distribution of Candida species in different clinical samples and their virulence: biofilm formation, proteinase and phospholipase production: A study on hospitalized patients in southern India. J Glob Infect Dis. Jan- Mar; 3(1): 4–8.

Samaranayake, Y. H.; Dassanayake, R. S. and Jayatilake, J. A. M. S. (2005). Phospholipase B enzyme expression is not associated with other virulence attributes in Candida albicans isolates from patients with human immunodeficiency virus infection. J Med Microbiol. 54: 583-93.

Ying, S. and Chunyang, L. (2012). Correlation between phospholipase of Candida albicans and resistance to fluconazole. Mycoses.55:50-55.

Hussain Owaied Wasmi Al-Juboory, Y. (2018). Utilization of Hydrocarbons of Two Types of Crude Oil By the Action of One Species of Bacteria and Two Species of Fungi and Synergism between them. Al-Kufa University Journal for Biology, 10(1). Retrieved from https: //journal. iq/ index. php/ ajb /article/ view/8206




How to Cite

razzaq majjed, T., Adel, M., & Al-Amer, A. (2023). Detection of Candida species isolated from immunocompromised patients in AL-Najaf province: Detection of Candida species isolated from immunocompromised patients in AL-Najaf province. Al-Kufa University Journal for Biology, 15(2), 49–54.