Study Genetic Distances Amonge Nine Okra, (Abelmoschus, esculentus) genotypes UsingTen ISSR markers
DOI:
https://doi.org/10.36320/ajb/v12.i3.11787Keywords:
Okra, ISSR markers, Genetic diversity, DendrogramAbstract
Ten from molecular marker ISSRs, (Inter, - Simple, Sequence, Repeats.) were used to find genetic, diversity, genetic* relationship, and DNA* fingerprint* of nine Okra (Abelmoschus esculentus) genotypes. Primers varied among them in giving unique DNA fingerprints, primers (UBC-809, HB12, and HBS10) gave a unique fingerprint for one genotype of okra, while primers (844A, UBC808) give unique fingerprint for four genotypes. High genetic distance was (0.722) between Egypt and Hasnawia while low genetic distance was (0.074) between Hasnawia and Lahluba. Cluster analysis (Phylogenetic tree) grouped studied genotypes in to two main cluster, the first large one included six genotype(Mosulia , Houseagrl, Khnisiraa ,Hasnawia ,Lahluba and Batra) and other small one includes three genotype (Soutl,Egypt and Zasco seed).ISSR marker could reveal genetic relationship in studied genotypes according to their origin, thus it gave an excellent tool to breeding programs help breeders .
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Copyright (c) 2020 Melath Kadhum Farhood AL- Ghufaili, Taif Razaq Majid, Attyaf Jameel Thamir Al-Tamimi
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